Effect of Maggot on the IL-6 and IFN-Ɣ Expression of Koi Fish (Cyprinus carpio) Infected with Edwardsiella tarda

The increase in koi fish cultivation can lead to risks and caused problems. main problem is high disease attacks by the bacteria Edwardsiella tarda. One of alternatives control immunostimulant treatment with use maggot, Hermetia illucens Larvae, known as Black Soldier Fly (BSF). This study aims report effect maggot a substitute for feed formulations on immune system infected E. used descriptive method, quantitative approach IL-6 IFN-Ɣ expression through RT-PCR analysis survival rate (%). Identification tarda was carried out PCR test method at molecular weight 200 bp. density that death 50% population (LD50) 72 hours 107 cells/ml. highest spleen found sample C 28,844 lowest in-sample K (+) 26,096. liver 29,060 D 25,428. C, 28,844, D, 27,219. 26,795, 25,615. this reached 30%-100%. optimal amount substitution 75% (Treatment C) feed.